23/09/2021

HIV/AIDS and cancer exist as comorbidities, with malignancies previously associated with HIV/AIDS, also known as the AIDS-defining cancers (ADCs), having been documented to decrease, while the non-AIDS defining cancer (NADCs) are on the rise. Since cancer is a highly heterogeneous disease, precision oncology as the most effective cancer therapy is gaining attraction, and many newly developed drugs have been designed to identify particular molecular features that may help in the diagnosis and treatment of cancer.

Among these, biomarkers are specific and highly sensitive markers of varied molecular nature that can be found and measured in blood or tissue. Cancer biomarkers are discovered through using molecular and cellular methodologies that focus on disease and drug mechanisms, which indicate the interaction of novel therapies with their intended target or in the pathogenesis of the disease itself. Biomarkers consist of genomic and proteomic patterns, chromosomal abnormalities, epigenetic signatures, single genes or proteins. This causes biomarkers to play a significant role in cancer screening, early diagnosis, cancer stratification, efficacy prediction and adverse reactions, making them a powerful tool also in prognosis and treatment.

Regarding HIV-infected individuals, it is important to point out that they have an increased risk for developing cancer, mainly due to their immune system being suppressed, frequent coinfection with oncogenic viruses and an increase in risky behaviour such as poor lifestyle. Early detection of cancer biomarkers in HIV-positive individuals would be useful to identify patients at high risk of tumour development.

ADCs are fortunately on the decline since the introduction of the highly active antiretroviral treatment (HAART) in 1996. Three of them are hot topics for biomarker research:

Cervical cancer. It develops as a result of a persistent infection with high-risk human papillomavirus (hrHPV) types, resulting in premalignant precursor lesions also known as cervical intraepithelial neoplasia (CIN). Grading of CIN lesions is vital for clinical management of patients and specific biomarkers are required for accurate grading, diagnosis, and ultimately effective treatment of CIN. Novel biomarkers for the diagnosis of cervical cancer include, among others, the presence of HPV E6/E7 mRNA, since the initiation and mediation of the oncogenic process of cervical cancer occurs by the upregulation of HPV E6/E7 oncoproteins. The overexpression of these oncoproteins serves as a biomarker for increased cervical cancer risk.

Non-Hodgkin Lymphoma (NHL). It is the second most common malignancy in HIV-infected patients and is characterised by diffused large B-cell lymphoma (DLBCL). The quality and quantity of affected lymph nodes for assessing morphology and architecture are the first requirement in the diagnosis of NHL. Blood count, white blood cells or platelet count tests are followed by pathological tests such as flow cytometry or immunohistochemistry. Protein expression biomarker Ki-67 or its antibody MIB-1 staining are used to identify aggressive lymphomas as these may be indicating a high growth fraction of tumours. Ki-67 expression in DLBCL patients is associated with poor outcome and survival.

Lastly, HIV-associated Kaposi’s Sarcoma (HIV-KS) is a low-grade vascular tumour which is associated with human herpesvirus-8 (HHV8)/KS-associated herpes virus (KSHV) infection, and is the most frequent and aggressive type of endothelial neoplasia. Lesion progression represents the expression of HHV8 latency that include factors such as latent nuclear antigen-1 (LANA-1), cyclin-D1 and bcl-2. The immunohistochemical detection of HHV8 of fixed tissues might be a diagnostic tool to differentiate KS.

Personalised medicine is the trend in cancer and in many other diseases. Its core depends on the discovery and the development of biomarkers and, in the case of HIV, a careful selection of sensitive and specific HIV-associated cancer biomarkers is key to identify patients at most risk of tumour development. These advancements will help improve the diagnosis, prognosis, and ultimately treatment of the disease, which will translate into a better quality of life for HIV-associated cancer patients.

 

Reference:

Dlamini Z, Mbele M, Makhafola TJ, et al. HIV-Associated Cancer Biomarkers: A Requirement for Early Diagnosis. Int J Mol Sci. 2021; 22(15): 8127.

 



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